ABSTRACT
Objective: To research the effect and mechanism of how chitosan restrain fat-absorbtion, and provide references for developing a kind of nutritional and healthy fastfood. Methods: 1.In vitro digestion: Phosphomolybdic acid colorimetry; 2.In vivo experiment:240 rats were divided into four groups and fed with different diets: basal diet(Group A), high fat diet (Group B), chitosan supplemented diet (Group C), high fat and chitosan supplemented diet (Group D). The fat and chitosan content(%) of diet were A(10,0), B(60,0), C(8,20), D(34.3,14.8) respectively. Determine the blood lipids and fat output in dung, and observe the weight gain and swimming time. Results: 1.In vitro experiment showed chitosan acted on cholic acid at the best proportion 1∶8. 2.In vivo experiment showed chitosan can restrain fat-absorbtion, and increase fat output. Swimming in ice-water showed chitosan improved rats performance. Compared with group B, the blood lipids and weight of group C and D had no obvious change, but the fat-output much increased.